In vivo carbamylation and acetylation of water-soluble human lens αB-crystallin lysine 92
نویسندگان
چکیده
منابع مشابه
Correction: In Vivo Substrates of the Lens Molecular Chaperones αA-Crystallin and αB-Crystallin
αA-crystallin and αB-crystallin are members of the small heat shock protein family and function as molecular chaperones and major lens structural proteins. Although numerous studies have examined their chaperone-like activities in vitro, little is known about the proteins they protect in vivo. To elucidate the relationships between chaperone function, substrate binding, and human cataract forma...
متن کاملαA-crystallin and αB-crystallin reside in separate subcellular compartments in the developing ocular lens.
αA-Crystallin (αA) and αB-crystallin (αB), the two prominent members of the small heat shock family of proteins are considered to be two subunits of one multimeric protein, α-crystallin, within the ocular lens. Outside of the ocular lens, however, αA and αB are known to be two independent proteins, with mutually exclusive expression in many tissues. This dichotomous view is buoyed by the high e...
متن کاملUV-A-induced structural and functional changes in human lens deamidated αB-crystallin
PURPOSE To determine comparative effects of ultraviolet (UV)-A irradiation on structural and functional properties of wild type (WT) alphaB-crystallin and its three deamidated mutant proteins (alphaB-Asn78Asp, alphaB-Asn146Asp, and alphaB-Asn78/146Asp). METHODS Three deamidated mutants previously generated from recombinant WT alphaB-crystallin, using a site-specific mutagenesis procedure as p...
متن کاملDown regulation of the PEDF gene in human lens epithelium cells changed the expression of proteins vimentin and αB-crystallin
PURPOSE To study the relationship of pigment epithelium-derived factor (PEDF) expression with the expression of vimentin and alphaB-crystallin by lens epithelial cells. METHODS Lens epithelial cells adhering to anterior capsules taken from young donor eyes aged from 20 to 35 years were cultured and passaged. We designed small interfering RNA (siRNA) constructs to specifically downregulate the...
متن کاملComparative Analysis of αB-Crystallin Expression in Heat-Stressed Myocardial Cells In Vivo and In Vitro
Relationships between αB-crystallin expression patterns and pathological changes of myocardial cells after heat stress were examined in vitro and in vivo in this study using the H9C2 cell line and Sprague-Dawley rats, respectively. Histopathological lesions, characterized by acute degeneration, karyopyknosis and loss of a defined nucleus, became more severe in rat hearts over the course of heat...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2001
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.40901